Tomato hybrid sv4725td and parents thereof

ABSTRACT

The invention provides seed and plants of tomato hybrid SV4725TD and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of tomato hybrid SV4725TD and the parent lines thereof, and to methods for producing a tomato plant produced by crossing such plants with themselves or with another tomato plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and, morespecifically, to the development of tomato hybrid SV4725TD and theinbred tomato lines FDR-9Q08131 and FDR-9Q09139.

BACKGROUND OF THE INVENTION

The goal of vegetable breeding is to combine various desirable traits ina single variety/hybrid. Such desirable traits may include any traitdeemed beneficial by a grower and/or consumer, including greater yield,resistance to insects or disease, tolerance to environmental stress, andnutritional value.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same plant or plant variety. A plant cross-pollinates if pollencomes to it from a flower of a different plant variety.

Plants that have been self-pollinated and selected for type over manygenerations become homozygous at almost all gene loci and produce auniform population of true breeding progeny, a homozygous plant. A crossbetween two such homozygous plants of different genotypes produces auniform population of hybrid plants that are heterozygous for many geneloci. Conversely, a cross of two plants each heterozygous at a number ofloci produces a population of hybrid plants that differ genetically andare not uniform. The resulting non-uniformity makes performanceunpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants, and theevaluation of the crosses. Pedigree breeding and recurrent selection areexamples of breeding methods that have been used to develop inbredplants from breeding populations. Those breeding methods combine thegenetic backgrounds from two or more plants or various other broad-basedsources into breeding pools from which new lines and hybrids derivedtherefrom are developed by selfing and selection of desired phenotypes.The new lines and hybrids are evaluated to determine which of those havecommercial potential.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides a tomato plant of thehybrid designated SV4725TD, the tomato line FDR-9Q08131 or tomato lineFDR-9Q09139. Also provided are tomato plants having all thephysiological and morphological characteristics of such a plant. Partsof these tomato plants are also provided, for example, including pollen,an ovule, scion, a rootstock, a fruit, and a cell of the plant.

In another aspect of the invention, a plant of tomato hybrid SV4725TDand/or tomato lines FDR-9Q08131 and FDR-9Q09139 comprising an addedheritable trait is provided. The heritable trait may comprise a geneticlocus that is, for example, a dominant or recessive allele. In oneembodiment of the invention, a plant of tomato hybrid SV4725TD and/ortomato lines FDR-9Q08131 and FDR-9Q09139 is defined as comprising asingle locus conversion. In specific embodiments of the invention, anadded genetic locus confers one or more traits such as, for example,herbicide tolerance, insect resistance, disease resistance, and modifiedcarbohydrate metabolism. In further embodiments, the trait may beconferred by a naturally occurring gene introduced into the genome of aline by backcrossing, a natural or induced mutation, or a transgeneintroduced through genetic transformation techniques into the plant or aprogenitor of any previous generation thereof. When introduced throughtransformation, a genetic locus may comprise one or more genesintegrated at a single chromosomal location.

The invention also concerns the seed of tomato hybrid SV4725TD and/ortomato lines FDR-9Q08131 and FDR-9Q09139. The tomato seed of theinvention may be provided as an essentially homogeneous population oftomato seed of tomato hybrid SV4725TD and/or tomato lines FDR-9Q08131and FDR-9Q09139. Essentially homogeneous populations of seed aregenerally free from substantial numbers of other seed. Therefore, insome embodiments, seed of hybrid SV4725TD and/or tomato linesFDR-9Q08131 and FDR-9Q09139 may be defined as forming at least about 97%of the total seed, including at least about 98%, 99% or more of theseed. The seed population may be separately grown to provide anessentially homogeneous population of tomato plants designated SV4725TDand/or tomato lines FDR-9Q08131 and FDR-9Q09139.

In yet another aspect of the invention, a tissue culture of regenerablecells of a tomato plant of hybrid SV4725TD and/or tomato linesFDR-9Q08131 and FDR-9Q09139 is provided. The tissue culture willpreferably be capable of regenerating tomato plants capable ofexpressing all of the physiological and morphological characteristics ofthe starting plant, and of regenerating plants having substantially thesame genotype as the starting plant. Examples of some of thephysiological and morphological characteristics of the hybrid SV4725TDand/or tomato lines FDR-9Q08131 and FDR-9Q09139 include those traits setforth in the tables herein. The regenerable cells in such tissuecultures may be derived, for example, from embryos, meristems,cotyledons, pollen, leaves, anthers, roots, root tips, pistils, flowers,seed and stalks. Still further, the present invention provides tomatoplants regenerated from a tissue culture of the invention, the plantshaving all the physiological and morphological characteristics of hybridSV4725TD and/or tomato lines FDR-9Q08131 and FDR-9Q09139.

In still yet another aspect of the invention, processes are provided forproducing tomato seeds, plants and fruit, which processes generallycomprise crossing a first parent tomato plant with a second parenttomato plant, wherein at least one of the first or second parent tomatoplants is a plant of tomato line FDR-9Q08131 or tomato line FDR-9Q09139.These processes may be further exemplified as processes for preparinghybrid tomato seed or plants, wherein a first tomato plant is crossedwith a second tomato plant of a different, distinct genotype to providea hybrid that has, as one of its parents, a plant of tomato lineFDR-9Q08131 or tomato line FDR-9Q09139. In these processes, crossingwill result in the production of seed. The seed production occursregardless of whether the seed is collected or not.

In one embodiment of the invention, the first step in “crossing”comprises planting seeds of a first and second parent tomato plant,often in proximity so that pollination will occur for example, mediatedby insect vectors. Alternatively, pollen can be transferred manually.Where the plant is self-pollinated, pollination may occur without theneed for direct human intervention other than plant cultivation.

A second step may comprise cultivating or growing the seeds of first andsecond parent tomato plants into plants that bear flowers. A third stepmay comprise preventing self-pollination of the plants, such as byemasculating the flowers (i.e., killing or removing the pollen).

A fourth step for a hybrid cross may comprise cross-pollination betweenthe first and second parent tomato plants. Yet another step comprisesharvesting the seeds from at least one of the parent tomato plants. Theharvested seed can be grown to produce a tomato plant or hybrid tomatoplant.

The present invention also provides the tomato seeds and plants producedby a process that comprises crossing a first parent tomato plant with asecond parent tomato plant, wherein at least one of the first or secondparent tomato plants is a plant of tomato hybrid SV4725TD and/or tomatolines FDR-9Q08131 and FDR-9Q09139. In one embodiment of the invention,tomato seed and plants produced by the process are first generation (F₁)hybrid tomato seed and plants produced by crossing a plant in accordancewith the invention with another, distinct plant. The present inventionfurther contemplates plant parts of such an F₁ hybrid tomato plant, andmethods of use thereof. Therefore, certain exemplary embodiments of theinvention provide an F₁ hybrid tomato plant and seed thereof.

In still yet another aspect, the present invention provides a method ofproducing a plant derived from hybrid SV4725TD and/or tomato linesFDR-9Q08131 and FDR-9Q09139, the method comprising the steps of: (a)preparing a progeny plant derived from hybrid SV4725TD and/or tomatolines FDR-9Q08131 and FDR-9Q09139, wherein said preparing comprisescrossing a plant of the hybrid SV4725TD and/or tomato lines FDR-9Q08131and FDR-9Q09139 with a second plant; and (b) crossing the progeny plantwith itself or a second plant to produce a seed of a progeny plant of asubsequent generation. In further embodiments, the method mayadditionally comprise: (c) growing a progeny plant of a subsequentgeneration from said seed of a progeny plant of a subsequent generationand crossing the progeny plant of a subsequent generation with itself ora second plant; and repeating the steps for an additional 3-10generations to produce a plant derived from hybrid SV4725TD and/ortomato lines FDR-9Q08131 and FDR-9Q09139. The plant derived from hybridSV4725TD and/or tomato lines FDR-9Q08131 and FDR-9Q09139 may be aninbred line, and the aforementioned repeated crossing steps may bedefined as comprising sufficient inbreeding to produce the inbred line.In the method, it may be desirable to select particular plants resultingfrom step (c) for continued crossing according to steps (b) and (c). Byselecting plants having one or more desirable traits, a plant derivedfrom hybrid SV4725TD and/or tomato lines FDR-9Q08131 and FDR-9Q09139 isobtained which possesses some of the desirable traits of the line/hybridas well as potentially other selected traits.

In certain embodiments, the present invention provides a method ofproducing food or feed comprising: (a) obtaining a plant of tomatohybrid SV4725TD and/or tomato lines FDR-9Q08131 and FDR-9Q09139, whereinthe plant has been cultivated to maturity, and (b) collecting at leastone tomato from the plant.

In still yet another aspect of the invention, the genetic complement oftomato hybrid SV4725TD and/or tomato lines FDR-9Q08131 and FDR-9Q09139is provided. The phrase “genetic complement” is used to refer to theaggregate of nucleotide sequences, the expression of which sequencesdefines the phenotype of, in the present case, a tomato plant, or a cellor tissue of that plant. A genetic complement thus represents thegenetic makeup of a cell, tissue or plant, and a hybrid geneticcomplement represents the genetic make up of a hybrid cell, tissue orplant. The invention thus provides tomato plant cells that have agenetic complement in accordance with the tomato plant cells disclosedherein, and seeds and plants containing such cells.

Plant genetic complements may be assessed by genetic marker profiles,and by the expression of phenotypic traits that are characteristic ofthe expression of the genetic complement, e.g., isozyme typing profiles.It is understood that hybrid SV4725TD and/or tomato lines FDR-9Q08131and FDR-9Q09139 could be identified by any of the many well knowntechniques such as, for example, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990),Randomly Amplified Polymorphic DNAs (RAPDs), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified FragmentLength Polymorphisms (AFLPs) (EP 534 858, specifically incorporatedherein by reference in its entirety), and Single NucleotidePolymorphisms (SNPs) (Wang et al., Science, 280:1077-1082, 1998).

In still yet another aspect, the present invention provides hybridgenetic complements, as represented by tomato plant cells, tissues,plants, and seeds, formed by the combination of a haploid geneticcomplement of a tomato plant of the invention with a haploid geneticcomplement of a second tomato plant, preferably, another, distincttomato plant. In another aspect, the present invention provides a tomatoplant regenerated from a tissue culture that comprises a hybrid geneticcomplement of this invention.

Any embodiment discussed herein with respect to one aspect of theinvention applies to other aspects of the invention as well, unlessspecifically noted.

The term “about” is used to indicate that a value includes the standarddeviation of the mean for the device or method being employed todetermine the value. The use of the term “or” in the claims is used tomean “and/or” unless explicitly indicated to refer to alternatives onlyor the alternatives are mutually exclusive. When used in conjunctionwith the word “comprising” or other open language in the claims, thewords “a” and “an” denote “one or more,” unless specifically notedotherwise. The terms “comprise,” “have” and “include” are open-endedlinking verbs. Any forms or tenses of one or more of these verbs, suchas “comprises,” “comprising,” “has,” “having,” “includes” and“including,” are also open-ended. For example, any method that“comprises,” “has” or “includes” one or more steps is not limited topossessing only those one or more steps and also covers other unlistedsteps. Similarly, any plant that “comprises,” “has” or “includes” one ormore traits is not limited to possessing only those one or more traitsand covers other unlisted traits.

Other objects, features and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and any specificexamples provided, while indicating specific embodiments of theinvention, are given by way of illustration only, since various changesand modifications within the spirit and scope of the invention willbecome apparent to those skilled in the art from this detaileddescription.

DETAILED DESCRIPTION OF THE INVENTION

The invention provides methods and compositions relating to plants,seeds and derivatives of tomato hybrid SV4725TD, tomato line FDR-9Q08131and tomato line FDR-9Q09139.

Tomato hybrid SV4725TD, also known as “EX 15584725”, is a fresh marketdeterminate round tomato variety widely adapted to growing conditions inthe Southeastern USA. Tomato hybrid features a strong plant type withgood fruit cover and a high percentage of extra large fruit with goodfruit quality. Hybrid SV4725TD is resistant to Alternaria alternata f.sp. lycopersici (Aal), Fusarium oxysporum f. sp. lycopersici US Races 1,2, 3 (Fol1, Fol2, Fol3), Meloidogyne incognita, Meloidogyne arenaria,Meloidogyne javanica (Ma/Mi/Mj), Tomato Spotted Wilt Virus (TSWV),Stemphylium solani (Ss), and Verticillium dahliae/Verticilliumalbo-atrum US race 1 (Va/Vd). Tomato hybrid SV4725TD provides addedresistance to Fusarium oxysporum f. sp. lycopersici US Races 1, 2, 3(Fol1, Fol2, Fol3), Meloidogyne incognita, Meloidogyne arenaria,Meloidogyne javanica (Ma/Mi/Mj) and Tomato Spotted Wilt Virus (TSWV)when compared commercial variety Florida 47R. Hybrid SV4725TD providesadded resistance to Meloidogyne incognita, Meloidogyne arenaria,Meloidogyne javanica and higher fruit quality when compared tocommercial variety BHN 602.

Hybrid SV4725TD is adapted for areas in Southeastern United States fromthe Eastern Shore of Virginia south to Central Florida where either orboth TSWV and Fol3 can be present. The variety also provides Ma/Mi/Mjnematode resistance that is becoming more important with changingfumigant procedures.

Line FDR-9Q08131 develops a large plant with good cover. It produces aheavy set of smooth, firm, flattened oblate extra large sized fruit. Thefruit have a jointed peduncle, uniform green shoulders and are red atmaturity. The line is resistant to nematode species Meloidogyneincognita, Meloidogyne arenaria, Meloidogyne javanica (Ma/Mi/Mj) andTomato Spotted Wilt Virus (TSWV). The line is also resistant toAlternaria alternata f. sp. lycopersici (Aal), Fusarium oxysporum f. sp.lycopersici US Races 1 and 2 (Fol1, Fol2), along with Verticilliumdahliae/Verticillium albo-atrum US race 1 (Va/Vd). It is believed thatthe most similar commercial variety is Florida 47R, but the lines differat least in that FDR-9Q08131 has resistance to Meloidogyne incognita,Meloidogyne arenaria, Meloidogyne javanica (Ma/Mi/Mj) and Tomato SpottedWilt Virus (TSWV), while Florida 47R is susceptible, and FDR-9Q08131 issusceptible to Stemphylium solani while Florida 47R is resistant.

Line FDR-9Q09139 develops a medium plant with light cover. It produces aheavy set of smooth, firm, deep oblate extra large sized fruit. Thefruit have a jointed pedicel, uniform green shoulders and are red atmaturity. The line is resistant to Fusarium oxysporum f. sp. lycopersiciUS Race 3 (Fol3). The line is also resistant to Alternaria alternata f.sp. lycopersici (Aal), Fusarium oxysporum f. sp. lycopersici US Races 1and 2 (Fol1, Fol2), along with Verticillium dahliae/Verticilliumalbo-atrum US race 1 (Va/Vd). It is believed that the most similarcommercial variety to FDR-9Q09139 is Florida 47R, but the lines differat least in that FDR-9Q09139 has resistance to Fusarium oxysporum f. sp.lycopersici US Race 3 (Fol3) while Florida 47R is susceptible.

A. ORIGIN AND BREEDING HISTORY OF TOMATO HYBRID SV4725TD

The parents of hybrid SV4725TD are FDR-9Q08131 and FDR-9Q09139. Theseparents were created as follows:

Line FDR-9Q08131, was also used to produce hybrid EX15567101 (U.S. Ser.No. 12/046,627) and hybrid EX15567631 (U.S. Ser. No. 13/283,453). LineFDR-9Q08131 was developed by pedigree selection from SVR 01522935, aSeminis hybrid first made in Woodland, Calif. This hybrid resulted fromthe cross between female FDR-15-2078 and male FDR-15-2079. The femaleparent, FDR-15-2079, develops a compact determinate plant and produces aheavy set of large to extra large deep globe red fruit. The parent isresistant to Alternaria alternata f. sp. lycopersici (Aal), Fusariumoxysporum f. sp. lycopersici US Races 1 and 2 (Fol1, Fol2), Meloidogyneincognita, Meloidogyne arenaria, Meloidogyne javanica (Ma/Mi/Mj),Stemphylium solani (Ss) and Verticillium dahliae/Verticillium albo-atrumUS race 1 (Va/Vd). The male parent, FDR-15-2078 develops a largedeterminate plant and produces medium to large deep oblate fruit. Theparent is resistant to Alternaria alternata f. sp. lycopersici (Aal),Fusarium oxysporum f. sp. lycopersici US Races 1 and 2 (Fol1, Fol2),Tomato Spotted Wilt Virus (TSWV), Tomato Yellow Leaf Curl Virus (TYLCV)and Verticillium dahliae/Verticillium albo-atrum US race 1 (Va/Vd).

Neither parent was marketed directly as an open pollinated line.FDR-9Q08131 differs from FDR-15-2079 at least because it has oblatefruit, is resistant to Tomato Spotted Wilt Virus (TSWV) and susceptibleto Stemphylium solani (Ss). FDR-9Q08131 differs from FDR-15-2078 atleast because it is resistant to Meloidogyne incognita, Meloidogynearenaria, Meloidogyne javanica (Ma/Mi/Mj), and susceptible to TomatoYellow Leaf Curl Virus (TYLCV).

The crossing and selections were made as follows:

-   -   August, Year 1—Plants of the F₁ SVR 01522935 were selfed in the        Seminis Woodland Calif. Station. Seed from the selfed plants was        bulked and shipped to Felda, Fla.    -   January, Year 2—F₂ population was planted in Felda, Fla. and        individual selections were made.    -   July, Year 2—F₃ line was planted. This selection had extra large        fruit and was fixed for Ma/Mi/Mj resistance and TSWV resistance        by molecular markers. Selections were made.    -   January, Year 3—F₄ line was planted. Selections were made.    -   January, Year 4—F₅ line was planted as stake. Selections were        made.    -   July, Year 4—F₆ line was planted. Selections were made.    -   January, Year 5—F₇ line was planted. Selections were made.    -   July, Year 5—F₈ line was planted. Selections were made. Line was        confirmed to be AF1F2VNSw< >TyS.    -   January, Year 6—F₉ line was planted. Selections were made.    -   July, Year 6—F₁₀ line was planted. The line was found to be        uniform and stable and selections were made.    -   January, Year 7—F₁₁ line was planted as finished line        FDR-9Q08131 and entered in the Foundation Seed increase.        Pathology tests confirmed resistance to Alternaria alternata f.        sp. lycopersici (Aal), Fusarium oxysporum f. sp. lycopersici US        Races 1 and 2 (Fol1, Fol2), Meloidogyne incognita, Meloidogyne        arenaria, Meloidogyne javanica (Ma/Mi/Mj) and Tomato Spotted        Wilt Virus (TSWV) and Verticillium dahliae/Verticillium        albo-atrum US race 1 (Va/Vd).

Parent line FDR-9Q09139 develops a medium plant with light cover. Itproduces a heavy set of smooth, firm, deep oblate extra large sizedfruit. The fruit have a jointed pedicel, uniform green shoulders and arered at maturity. The line is resistant to Fusarium oxysporum f. sp.lycopersici US Race 3 (Fol3). The line is also resistant to Alternariaalternata f. sp. lycopersici (Aal), Fusarium oxysporum f. sp.lycopersici US Races 1 and 2 (Fol1, Fol2), along with Verticilliumdahliae/Verticillium albo-atrum US race 1 (Va/Vd).

Tomato line FDR-9Q09139 was developed by pedigree selection fromSebring, a commercial hybrid from Syngenta.

The crossing and selections were made as follows:

-   -   October, Year 1: Plants of the F₁ Sebring were selfed in        Immokalee, Fla.    -   October, Year 2: F₂ population was planted in Felda, Fla. and        individual selections were made.    -   October, Year 3: F₃ line was planted. Selections were made.    -   October, Year 4: F₄ line was planted. The line was fixed for Fol        3 resistance based on molecular markers.    -   October, Year 5: F₅ line was planted. Selections were made.    -   January, Year 7: F₆ line was planted. The line was found to be        uniform and stable and selections were made.    -   April, Year 8: F₇ line was planted as finished line FDR-9Q09139        and entered into Foundation Seed increase. Pathology tests        confirmed resistance to Fusarium oxysporum f. sp. lycopersici US        Race 3 (Fol3), Alternaria alternata f. sp. lycopersici (Aal),        Fusarium oxysporum f. sp. lycopersici US Races 1 and 2 (Fol1,        Fol2) and Verticillium dahliae/Verticillium albo-atrum US race 1        (Va/Vd).

Parent line FDR-9Q09139 has been observed as uniform and stable over twogenerations and it is within commercially acceptable limits. As is truewith other tomato inbreds, a small percentage of variants can occurwithin commercially acceptable limits for almost any characteristicduring the course of repeated multiplication. However, no known variantswere observed during field trial observations or greenhouse crossingblock observations.

The parent lines are uniform and stable, as is a hybrid producedtherefrom. A small percentage of variants can occur within commerciallyacceptable limits for almost any characteristic during the course ofrepeated multiplication. However no variants are expected.

B. PHYSIOLOGICAL AND MORPHOLOGICAL CHARACTERISTICS OF TOMATO HYBRIDSV4725TD, TOMATO LINE FDR-9008131 AND TOMATO LINE FDR-9009139

In accordance with one aspect of the present invention, there isprovided a plant having the physiological and morphologicalcharacteristics of tomato hybrid SV4725TD and the parent lines thereof.A description of the physiological and morphological characteristics ofsuch plants is presented in Tables 1-3.

TABLE 1 Physiological and Morphological Characteristics of HybridSV4725TD Comparison: Characteristic SV4725TD FL 47 1. Seedlinganthocyanin in hypocotyl of 2-15 cm present present seedling (MontfavetH 63.4) habit of 3-4 week old seedling normal normal 2. Mature Plantheight 70.5 cm 129.3 cm growth type determinate determinate (Cambell1327, Prisca) Number of inflorescences on main medium medium stem (sideshoots to be removed) (Montfavet H 63.4) form normal normal size ofcanopy (compared to others of small medium similar type) habitsemi-erect semi-erect 3. Stem anthocyanin coloration of upper thirdabsent or very weak absent or very weak branching sparse intermediate(Brehm's Solid Red, (Westover) Fireball) branching at cotyledon or firstleafy present absent node number of nodes between first 1 to 4 7 to 10inflorescence number of nodes between early (1^(st) to 4 to 7 1 to 42^(nd), 2^(nd) to 3^(rd)) inflorescences number of nodes between later 1to 4 1 to 4 developing inflorescences pubescence on younger stemsmoderately hairy moderately hairy 4. Leaf type (mature leaf beneath the3^(rd) tomato Tomato inflorescence) margins of major leaflets (matureleaf shallowly toothed or shallowly toothed or beneath the 3^(rd)inflorescence) scalloped scalloped marginal rolling or wiltiness (maturemoderate moderate leaf beneath the 3^(rd) inflorescence) onset ofleaflet rolling midseason midseason surface of major leaflets (matureleaf rugose (bumpy or rugose (bumpy or beneath the 3^(rd) inflorescence)veiny) veiny) pubescence (mature leaf beneath the normal normal 3^(rd)inflorescence) attitude (in middle third of plant) semi-erect (Allround,semi-erect Drakar, Vitador) (Allround, Drakar, Vitador) length shortlong (Nelson, Red Robin, Tiny Tim) width narrow medium (Marmande VR, RedRobin, Tiny Tim) division of blade pinnate pinnate (Mikado, Pilot, Red(Mikado, Pilot, Red Jacket) Jacket) size of leaflets (in middle of leaf)small medium (Marmande (Tiny Tim) VR, Royesta) intensity of green colormedium (Lucy) dark (Allround, Daniela, Lorena, Red Robin) glossiness (asfor 6) weak (Daniela) weak (Daniela) blistering (as for 6) weak(Daniela) strong (Delfine, Tiny Tim) size of blisters (as for 6) smallmedium (Husky Cherrie Red) (Marmande VR) attitude of petiole of leafletin relation semi-drooping semi-erect (Blizzard, to main axis (in middleof leaf) (Montfavet H 63.5) Marmande VR) 5. Inflorescence inflorescencetype (2^(nd) and 3^(rd) truss) mainly uniparous intermediate (Dynamo)(Harzfeuer) type (3^(rd) inflorescence) simple forked (2 major axes)average number of flowers in 3.9 5 inflorescence (3^(rd) inflorescence)leafy or “running” inflorescence (3^(rd) absent occasionalinflorescence) 6. Flower calyx normal (lobes awl normal shaped)calyx-lobes shorter than corolla shorter than corolla corolla coloryellow yellow style pubescence absent or very scarce dense/present(Campbell 1327) (Saint-Pierre) anthers all fused into tube all fusedinto tube fasciation (1^(st) flower of 2^(nd) or 3^(rd) absent (Monalbo,absent (Monalbo, inflorescence) Moneymaker) Moneymaker) color yellowyellow (Marmande VR) 7. Fruit typical shape in longitudinal sectionslightly flattened slightly flattened (3^(rd) fruit of 2^(nd) or 3^(rd)cluster) shape of transverse/cross section (3^(rd) round round fruit of2^(nd) or 3^(rd) cluster) shape of stem end (3^(rd) fruit of 2^(nd) or3^(rd) indented indented cluster) shape of blossom end (3^(rd) fruit of2^(nd) or flat flat 3^(rd) cluster) (Montfavet H 63.4, Montfavet H 63.5)size of blossom scar small small (Montfavet H 63.4, Montfavet H 63.5)shape of pistil scar (3^(rd) fruit of 2^(nd) or irregular dot 3^(rd)cluster) peduncle: abscission layer (3^(rd) fruit of present(pedicellate) present 2^(nd) or 3^(rd) cluster) (Montfavet H 63.5, Romapeduncle: length (from abscission layer medium (Dario, medium to calyx)(only for varieties with Primosol) abscission layers) ribbing atpeduncle end medium medium (Montfavet H 63.4, Montfavet H 63.5)depression at peduncle end medium weak (Carmello, Count, Fandango,Saint- Pierre) size of stem/peduncle scar large medium (Apla, Campbell1327, Carmello, Fandango, Floradade) point of detachment of fruit atharvest at pedicel joint at pedicel joint (3^(rd) fruit of 2^(nd) or3^(rd) cluster) length of dedicel (3^(rd) fruit of 2^(nd) or 3^(rd) 14.2 mm  12.9 mm cluster) length of mature fruit (stem axis; 3^(rd) 71.6 mm  60.3 mm fruit of 2^(nd) or 3^(rd) cluster) diameter of fruitat widest point (3^(rd)  84.8 mm  67.1 mm fruit of 2^(nd) or 3^(rd)cluster) weight of mature fruit (3^(rd) fruit of 2^(nd) 323.4 grams166.8 grams or 3^(rd) cluster) size very large large (Erlidor, Lydia,Muril) ratio length/diameter medium (Early Mech, medium Peto Gro) corepresent present size of core in cross section (in relation very largelarge to total diameter) (Marmande VR, Valenciano) number of loculesMore than 6 4, 5 or 6 (Marmande VR) surface slightly rough smooth basecolor (mature-green stage) light green red (Lanai, VF 145-F5) pattern(mature-green stage) uniform green uniform green green shoulder (beforematurity) absent absent (Felicia, Rio Grande, Trust) intensity of greencolor of fruit (before light light maturity) (Capello, Duranto, Trust)color at maturity (full-ripe) red red (Ferline, Daniela, Montfavet H63.5) color of flesh at maturity (full-ripe) red/crimson red/crimson(Ferline, Saint-Pierre) flesh color uniform uniform locular gel color oftable-ripe fruit red red firmness soft (Trend) medium (Cristina) shelflife short (Rambo) short time of flowering medium medium (Montfavet H63.5, Prisca) time of maturity medium medium (Montfavet H 63.5) ripening(blossom-to-stem axis) uniform uniform ripening (peripheral to centralradial uniformity uniformity axis) epidermis color yellow yellowepidermis normal normal epidermis texture average tender thickness ofpericarp thin thick (Marmanade VR) dry matter content (at maturity) lowmedium (Bonset) sensitivity to silvering insensitive insensitive(Marathon, Sano) (Marathon, Sano) 8. Chemistry and Composition ofFull-Ripe Fruits pH  4.37  4.3 titratable acidity, as % citric  0.318 0.397 total solids (dry matter, seeds and skin  4.7  5.7 removed)soluble Solids as °Brix  4.4  5.3 9. Phenology seeding to 50% flow (1open on 50%  49.5  53.5 of plants) seeding to once over harvest (if 120120 applicable) fruiting season very concentrated (UC 82) Relativematurity in areas tested medium early 10. Adaptation culture fieldprinciple use(s) home garden, fresh market machine harvest not adaptedregions to which adaptation has been California; demonstrated Sacramentoand Upper San Joaquin *These are typical values. Values may vary due toenvironment. Other values that are substantially equivalent are alsowithin the scope of the invention.

TABLE 2 Physiological and Morphological Characteristics of LineFDR-9Q08131 Comparison: Characteristic FDR-9Q08131 FL47 1. Seedlinganthocyanin in hypocotyl of 2-15 cm present (Montfavet present seedlingH 63.4) habit of 3-4 week old seedling normal normal 2. Mature Plantheight 57.4 cm 70.2 cm growth type determinate determinate (Campbell1327, Prisca) plant: number of inflorescences on main medium (Montfavetmedium stem (side shoots to be removed) H 63.4) form compact lax, opensize of canopy (compared to others of small large similar type) habiterect (Dwarf sprawling Champion) 3. Stem anthocyanin coloration of upperthird absent or very weak absent or very weak branching intermediatesparse (Westover) branching at cotyledon or first leafy node absentpresent number of nodes between first inflorescence 4 to 7 4 to 7 numberof nodes between early (1^(st) to 2^(nd), 1 to 4 4 to 7 2^(nd) to3^(rd)) inflorescences number of nodes between later developing 4 to 7 7to 10 inflorescences pubescence on younger stems sparsely hairy sparselyhairy (scattered long hairs) 4. Leaf type (mature leaf beneath the3^(rd) tomato tomato inflorescence) morphology (mature leaf beneath the3^(rd) pinnate leaf with pinnate leaf with inflorescence) medium sizedmedium sized leaflets leaflets margins of major leaflets (mature leafshallowly toothed nearly entire beneath the 3^(rd) inflorescence) orscalloped marginal rolling or wiltiness (mature leaf absent absentbeneath the 3^(rd) inflorescence) surface of major leaflets (mature leafrugose (bumpy or smooth beneath the 3^(rd) inflorescence) veiny)pubescence (mature leaf beneath the 3^(rd) hirsute normal inflorescence)attitude (in middle third of plant) semi-erect horizontal (Allround,Drakar, Vitador) length long (Montfavet H medium 63.5) width mediummedium division of blade pinnate (Mikado, pinnate Pilot, Red Jacket)size of leaflets (in middle of leaf) medium medium (Marmande VR,Royesta) intensity of green color medium (Lucy) dark glossiness (as for6) weak (Daniela) medium blistering (as for 6) strong (Delfine, mediumTiny Tim) size of blisters (as for 6) small (Husky medium Cherrie Red)attitude of petiole of leaflet in relation to horizontal horizontal mainaxis (in middle of leaf) (Sonatine) 5. Inflorescence inflorescence type(2^(nd) and 3^(rd) truss) intermediate mainly uniparous (Harzfeuer) type(3^(rd) inflorescence) forked (2 major forked axes) average number offlowers in inflorescence 4.2 4.8 (3^(rd) inflorescence) leafy or“running” inflorescence (3^(rd) occasional occasional inflorescence) 6.Flower calyx normal (lobes awl normal shaped) calyx-lobes shorter thancorolla shorter than corolla corolla color yellow yellow stylepubescence absent or very absent or very scarce (Campbell scarce 1327)anthers all fused into tube all fused into tube fasciation (1^(st)flower of 2^(nd) or 3^(rd) absent (Monalbo, absent inflorescence)Moneymaker) color yellow (Marmande yellow VR) 7. Fruit typical shape inlongitudinal section (3^(rd) slightly flattened slightly flattened fruitof 2^(nd) or 3^(rd) cluster) shape of transverse/cross section (3^(rd)fruit angular irregular of 2^(nd) or 3^(rd) cluster) shape of stem end(3^(rd) fruit of 2^(nd) or 3^(rd) indented indented cluster) shape ofblossom end (3^(rd) fruit of 2^(nd) or 3^(rd) flat to pointed/ indentedto flat cluster) nippled (Cal J, Early Mech, Peto Gro) size of blossomscar small (Montfavet H medium 63.4, Montfavet H 63.5) shape of pistilscar (3^(rd) fruit of 2^(nd) or 3^(rd) linear stellate cluster)peduncle: abscission layer (3^(rd) fruit of 2^(nd) or present present3^(rd) cluster) (pedicellate) (Montfavet H 63.5, Roma) varieties withabscission layers: Peduncle: short (Cerise, short length from abscissionlayer to calyx Ferline, Montfavet H 63.18, Rossol) ribbing at peduncleend medium (Montfavet strong H 63.4, Montfavet H 63.5) depression atpeduncle end weak (Futuria, medium Melody) size of stem/peduncle scarlarge (Apla, large Campbell 1327, Carmello, Fandango, Floradade) pointof detachment of fruit at harvest (3^(rd) at pedicel joint at pediceljoint fruit of 2^(nd) or 3^(rd) cluster) length of dedicel (3^(rd) fruitof 2^(nd) or 3^(rd) 10.9 mm 12.8 mm cluster) length of mature fruit(stem axis; 3^(rd) fruit of 64.6 65.8 mm 2^(nd) or 3^(rd) cluster)diameter of fruit at widest point (3^(rd) fruit of 72.3 mm  72.5 mm2^(nd) or 3^(rd) cluster) weight of mature fruit (3^(rd) fruit of 2^(nd)or 3^(rd)  192 grams 210.8 grams cluster) size large (Carmello, largeRingo) ratio length/diameter medium (Early large Mech, Peto Gro) corecoreless (absent or coreless smaller than 6 × 6 mm) number of locules 4,5 or 6 (Raïssa, more than 6 Tradiro) surface smooth smooth base color(mature-green stage) light green (Lanai, yellow green VF 145-F5) pattern(mature-green stage) uniform green uniform green green shoulder (beforematurity) absent (Felicia, Rio absent Grande, Trust) intensity of greencolor of fruit (before light (Capello, light maturity) Duranto, Trust)color at maturity (full-ripe) red (Ferline, red Daniela, Montfavet H63.5) color of flesh at maturity (full-ripe) red/crimson red crimson(Ferline, Saint- Pierre) flesh color uniform uniform locular gel colorof table-ripe fruit yellow red firmness medium (Cristina) soft shelflife short (Rambo) short time of flowering medium (Montfavet medium H63.5, Prisca) time of maturity medium (Montfavet medium H 63.5) ripening(blossom-to-stem axis) blossom-to-stem uniform end ripening (peripheralto central radial axis) uniformity uniformity epidermis color yellowyellow epidermis normal normal epidermis texture average averagethickness of pericarp medium (Carmello, thin Europeel, Floradade, Heinz1706, Montfavet H 63.5) dry matter content (at maturity) low (Bonset)medium sensitivity to silvering insensitive insensitive (Marathon, Sano)8. Chemistry and Composition of Full-Ripe Fruits pH 4.22 4.32 titratableAcidity, as % citric 0.462 0.48 total solids (dry matter, seeds and skin5.38 6.03 removed, expressed as % residue on wt per wt basis) solubleSolids as °Brix 4.57 4.96 9. Phenology seeding to 50% flow (1 open on50% of  55 days  55 days plants) seeding to once over harvest (ifapplicable) 127 days 120 days fruiting season long (Marglobe) relativematurity in areas tested medium 10. Adaptation culture field principleuse(s) fresh market machine harvest not adapted regions to whichadaptation has been Sacramento and demonstrated Upper San Joaquin valleyof California *These are typical values. Values may vary due toenvironment. Other values that are substantially equivalent are alsowithin the scope of the invention.

TABLE 3 Physiological and Morphological Characteristics of LineFDR-9Q09139 Comparison Variety Characteristic FDR-9Q09139 FL47 1.Seedling anthocyanin in hypocotyl of present present 2-15 cm seedling(Montfavet H 63.4) habit of 3-4 week old normal normal seedling 2.Mature Plant height 63.5 cm 69.65 cm growth type determinate determinate(Campbell 1327, Prisca) number of inflorescences on few (Campbell 1327)medium main stem (side shoots to be removed) form normal lax, open sizeof canopy (compared to medium large others of similar type) habitsemi-erect sprawling 3. Stem anthocyanin coloration of absent or veryweak absent or very weak upper third branching sparse sparse (Brehm'sSolid Red, Fireball) branching at cotyledon or present present firstleafy node number of nodes between 7 to 10 4 to 7 first inflorescencenumber of nodes between 1 to 4 4 to 7 early (1^(st) to 2^(nd), 2^(nd) to3^(rd)) inflorescences number of nodes between 1 to 4 7 to 10 laterdeveloping inflorescences pubescence on younger stems sparsely hairysparsely hairy (scattered long hairs) 4. Leaf type (mature leaf beneaththe tomato tomato 3^(rd) inflorescence) margins of major leafletsshallowly toothed or nearly entire (mature leaf beneath the 3^(rd)scalloped inflorescence) marginal rolling or wiltiness moderate absent(mature leaf beneath the 3^(rd) inflorescence) onset of leaflet rollingmidseason (mature leaf beneath the 3rd inflorescence) surface of majorleaflets rugose (bumpy or veiny) smooth (mature leaf beneath the 3^(rd)inflorescence) pubescence (mature leaf normal normal beneath the 3^(rd)inflorescence) attitude (in middle third of horizontal horizontal plant)(Aromata, Triton) length medium medium (Lorena) width medium mediumdivision of blade pinnate (Mikado, Pilot, pinnate Red Jacket) size ofleaflets (in middle of medium medium leaf) (Marmande VR, Royesta)intensity of green color dark dark (Allround, Daniela, Lorena, RedRobin) glossiness (as for 6) weak (Daniela) medium blistering (as for 6)weak (Daniela) medium size of blisters (as for 6) small (Husky Cherriemedium Red) attitude of petiole of leaflet in horizontal (Sonatine)horizontal relation to main axis (as for 6) 5. Inflorescenceinflorescence type (2^(nd) and mainly multiparous mainly uniparous3^(rd) truss) (Marmande VR) type (3^(rd) inflorescence) simple forkedaverage number of flowers in 4.6 4.4 inflorescence (3^(rd)inflorescence) leafy or “running” occasional occasional inflorescence(3^(rd) inflorescence) 6. Flower calyx normal (lobes awl shaped) normalcalyx-lobes shorter than corolla shorter than corolla corolla coloryellow yellow style pubescence sparse absent or very scarce anthers allfused into tube all fused into tube fasciation (1^(st) flower of 2^(nd)or absent (Monalbo, absent 3^(rd) inflorescence) Moneymaker) coloryellow (Marmande VR) yellow 7. Fruit typical shape in longitudinalslightly flattened slightly flattened section (3^(rd) fruit of 2^(nd) or3^(rd) cluster) shape of transverse/cross round irregular section(3^(rd) fruit of 2^(nd) or 3^(rd) cluster) shape of stem end (3^(rd)fruit of indented indented 2^(nd) or 3^(rd) cluster) shape of blossomend (3^(rd) indented to flat indented to flat fruit of 2^(nd) or 3^(rd)cluster) size of blossom scar medium medium (Alphamech, Apla, Carmello,Floradade) shape of pistil scar irregular stellate peduncle: abscissionlayer absent (jointless) present (3^(rd) fruit of 2^(nd) or 3^(rd)cluster) (Aledo, Bandera, Count, Lerica) ribbing at peduncle end absentor very weak strong (Calimero, Cerise) depression at peduncle end mediummedium (Carmello, Count, Fandango, Saint-Pierre) size of stem/pedunclescar large (Apla, Campbell large 1327, Carmello, Fandango, Floradade)point of detachment of fruit at calyx attachment at pedicel joint atharvest length of mature fruit (stem  70.3 mm  61.5 mm axis; 3^(rd)fruit of 2^(nd) or 3^(rd) cluster) diameter of fruit at widest  76.2 mm 68.4 mm point (3^(rd) fruit of 2^(nd) or 3^(rd) cluster) weight ofmature fruit (3^(rd) 218.9 grams 188.1 grams fruit of 2^(nd) or 3^(rd)cluster) size large (Carmello, Ringo) large ratio length/diameter largelarge (Rimone, Rio Grande) core present coreless size of core in crosssection medium (in relation to total diameter) (Montfavet H 63.4,Montfavet H 63.5) number of locules 4, 5 or 6 more than 6 (Raissa,Tradiro) surface smooth smooth base color (mature-green light green(Lanai, VF yellow green stage) 145-F5) pattern (mature-green stage)uniform green uniform green green shoulder (before absent absentmaturity) (Felicia, Rio Grande, Trust) intensity of green color of light(Capello, Duranto, light fruit (before maturity) Trust) color atmaturity (full-ripe) red red (Ferline, Daniela, Montfavet H 63.5) colorof flesh at maturity red/crimson (Ferline, red/crimson (full-ripe)Saint-Pierre) flesh color with lighter and darker uniform areas in wallslocular gel color of table-ripe red red fruit firmness medium (Cristina)soft shelf life short (Rambo) short time of flowering medium medium(Montfavet H 63.5, Prisca) time of maturity medium early (Montfavet H63.5) ripening uniform uniform ripening inside out uniformity epidermiscolor yellow yellow epidermis normal normal epidermis texture averageaverage thickness of pericarp thick thin (Cal J, Daniela, Ferline, PetoGro, Rio Grande) dry matter content (at medium medium maturity)sensitivity to silvering insensitive (Marathon, insensitive Sano) 8.Chemistry and Composition of Full-Ripe Fruits pH  4.35  4.29 titratableacidity, as % citric  0.33  0.458 total solids (dry matter, seeds  5.9 6.29 and skin removed) soluble Solids as °Brix  5.33  5.49 9. Phenologyseeding to 50% flow (1 open  62  59 on 50% of plants) seeding to onceover harvest 138 125 fruiting season medium (Westover) short,concentrated relative maturity in areas medium early tested 10.Adaptation culture field field principle use(s) home garden, fresh homegarden, fresh market market machine harvest not adapted not adaptedregions to which adaptation California: Sacramento has been demonstratedand Upper San Joaquin *These are typical values. Values may vary due toenvironment. Other values that are substantially equivalent are alsowithin the scope of the invention.

C. BREEDING TOMATO PLANTS

One aspect of the current invention concerns methods for producing seedof tomato hybrid SV4725TD involving crossing tomato lines FDR-9Q08131and FDR-9Q09139. Alternatively, in other embodiments of the invention,hybrid SV4725TD, line FDR-9Q08131, or line FDR-9Q09139 may be crossedwith itself or with any second plant. Such methods can be used forpropagation of hybrid SV4725TD and/or the tomato lines FDR-9Q08131 andFDR-9Q09139, or can be used to produce plants that are derived fromhybrid SV4725TD and/or the tomato lines FDR-9Q08131 and FDR-9Q09139.Plants derived from hybrid SV4725TD and/or the tomato lines FDR-9Q08131and FDR-9Q09139 may be used, in certain embodiments, for the developmentof new tomato varieties.

The development of new varieties using one or more starting varieties iswell known in the art. In accordance with the invention, novel varietiesmay be created by crossing hybrid SV4725TD followed by multiplegenerations of breeding according to such well known methods. Newvarieties may be created by crossing with any second plant. In selectingsuch a second plant to cross for the purpose of developing novel lines,it may be desired to choose those plants which either themselves exhibitone or more selected desirable characteristics or which exhibit thedesired characteristic(s) when in hybrid combination. Once initialcrosses have been made, inbreeding and selection take place to producenew varieties. For development of a uniform line, often five or moregenerations of selfing and selection are involved.

Uniform lines of new varieties may also be developed by way ofdouble-haploids. This technique allows the creation of true breedinglines without the need for multiple generations of selfing andselection. In this manner true breeding lines can be produced in aslittle as one generation. Haploid embryos may be produced frommicrospores, pollen, anther cultures, or ovary cultures. The haploidembryos may then be doubled autonomously, or by chemical treatments(e.g. colchicine treatment). Alternatively, haploid embryos may be growninto haploid plants and treated to induce chromosome doubling. In eithercase, fertile homozygous plants are obtained. In accordance with theinvention, any of such techniques may be used in connection with a plantof the invention and progeny thereof to achieve a homozygous line.

Backcrossing can also be used to improve an inbred plant. Backcrossingtransfers a specific desirable trait from one inbred or non-inbredsource to an inbred that lacks that trait. This can be accomplished, forexample, by first crossing a superior inbred (A) (recurrent parent) to adonor inbred (non-recurrent parent), which carries the appropriate locusor loci for the trait in question. The progeny of this cross are thenmated back to the superior recurrent parent (A) followed by selection inthe resultant progeny for the desired trait to be transferred from thenon-recurrent parent. After five or more backcross generations withselection for the desired trait, the progeny have the characteristicbeing transferred, but are like the superior parent for most or almostall other loci. The last backcross generation would be selfed to givepure breeding progeny for the trait being transferred.

The plants of the present invention are particularly well suited for thedevelopment of new lines based on the elite nature of the geneticbackground of the plants. In selecting a second plant to cross withSV4725TD and/or tomato lines FDR-9Q08131 and FDR-9Q09139 for the purposeof developing novel tomato lines, it will typically be preferred tochoose those plants which either themselves exhibit one or more selecteddesirable characteristics or which exhibit the desired characteristic(s)when in hybrid combination. Examples of desirable traits may include, inspecific embodiments, high seed yield, high seed germination, seedlingvigor, high fruit yield, disease tolerance or resistance, andadaptability for soil and climate conditions. Consumer-driven traits,such as a fruit shape, color, texture, and taste are other examples oftraits that may be incorporated into new lines of tomato plantsdeveloped by this invention.

D. PERFORMANCE CHARACTERISTICS

As described above, hybrid SV4725TD exhibits desirable traits, asconferred by tomato lines FDR-9Q08131 and FDR-9Q09139. The performancecharacteristics of hybrid SV4725TD and tomato lines FDR-9Q08131 andFDR-9Q09139 were the subject of an objective analysis of the performancetraits relative to other varieties.

TABLE 4 Performance data of hybrid SV4725TD and Comparative VarietiesWeight of Weight of Extra Number of Extra Large Number of Weight ofNumber of Large Fruit Extra Large Fruit Non- Extra Large AverageVariety-Average Extra Large Extra Large Marketable Fruit MarketableFruit Non- Weight across 3 trials Fruit (KG) Fruit (KG) Marketable (KG)Marketable (KG) SV4725TD 16.08 64.33 14.18 55.67 1.89 8.33 0.25 Florida47R 11.93 53.67 9.94 43.33 1.99 10.33 0.23 Florida 91 12.90 53.67 11.6348.00 1.27 5.67 0.24 Phoenix 14.97 62.33 12.93 53.00 2.04 9.33 0.24

TABLE 5 Performance data of hybrid SV4725TD and Comparative VarietiesVariety-Key Disease Resistance TSWV Fol3 Ma/Mi/Mj SV4725TD RES RES RESFlorida 47R SUS SUS SUS Florida 91 SUS SUS SUS Phoenix SUS SUS SUS

E. FURTHER EMBODIMENTS OF THE INVENTION

In certain aspects of the invention, plants described herein areprovided modified to include at least a first desired heritable trait.Such plants may, in one embodiment, be developed by a plant breedingtechnique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to a genetic locus transferred into the plant viathe backcrossing technique. The term single locus converted plant asused herein refers to those tomato plants which are developed by a plantbreeding technique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to the single locus transferred into the varietyvia the backcrossing technique. By essentially all of the morphologicaland physiological characteristics, it is meant that the characteristicsof a plant are recovered that are otherwise present when compared in thesame environment, other than an occasional variant trait that mightarise during backcrossing or direct introduction of a transgene.

Backcrossing methods can be used with the present invention to improveor introduce a characteristic into the present variety. The parentaltomato plant which contributes the locus for the desired characteristicis termed the nonrecurrent or donor parent. This terminology refers tothe fact that the nonrecurrent parent is used one time in the backcrossprotocol and therefore does not recur. The parental tomato plant towhich the locus or loci from the nonrecurrent parent are transferred isknown as the recurrent parent as it is used for several rounds in thebackcrossing protocol.

In a typical backcross protocol, the original variety of interest(recurrent parent) is crossed to a second variety (nonrecurrent parent)that carries the single locus of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a tomato plant isobtained wherein essentially all of the morphological and physiologicalcharacteristics of the recurrent parent are recovered in the convertedplant, in addition to the single transferred locus from the nonrecurrentparent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalvariety. To accomplish this, a single locus of the recurrent variety ismodified or substituted with the desired locus from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphologicalconstitution of the original variety. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross; one ofthe major purposes is to add some commercially desirable trait to theplant. The exact backcrossing protocol will depend on the characteristicor trait being altered and the genetic distance between the recurrentand nonrecurrent parents. Although backcrossing methods are simplifiedwhen the characteristic being transferred is a dominant allele, arecessive allele, or an additive allele (between recessive anddominant), may also be transferred. In this instance it may be necessaryto introduce a test of the progeny to determine if the desiredcharacteristic has been successfully transferred.

In one embodiment, progeny tomato plants of a backcross in which a plantdescribed herein is the recurrent parent comprise (i) the desired traitfrom the non-recurrent parent and (ii) all of the physiological andmorphological characteristics of tomato the recurrent parent asdetermined at the 5% significance level when grown in the sameenvironmental conditions.

New varieties can also be developed from more than two parents. Thetechnique, known as modified backcrossing, uses different recurrentparents during the backcrossing. Modified backcrossing may be used toreplace the original recurrent parent with a variety having certain moredesirable characteristics or multiple parents may be used to obtaindifferent desirable characteristics from each.

With the development of molecular markers associated with particulartraits, it is possible to add additional traits into an established germline, such as represented here, with the end result being substantiallythe same base germplasm with the addition of a new trait or traits.Molecular breeding, as described in Moose and Mumm, 2008 (PlantPhysiology, 147: 969-977), for example, and elsewhere, provides amechanism for integrating single or multiple traits or QTL into an eliteline. This molecular breeding-facilitated movement of a trait or traitsinto an elite line may encompass incorporation of a particular genomicfragment associated with a particular trait of interest into the eliteline by the mechanism of identification of the integrated genomicfragment with the use of flanking or associated marker assays. In theembodiment represented here, one, two, three or four genomic loci, forexample, may be integrated into an elite line via this methodology. Whenthis elite line containing the additional loci is further crossed withanother parental elite line to produce hybrid offspring, it is possibleto then incorporate at least eight separate additional loci into thehybrid. These additional loci may confer, for example, such traits as adisease resistance or a fruit quality trait. In one embodiment, eachlocus may confer a separate trait. In another embodiment, loci may needto be homozygous and exist in each parent line to confer a trait in thehybrid. In yet another embodiment, multiple loci may be combined toconfer a single robust phenotype of a desired trait.

Many single locus traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single locus traits may or may not betransgenic; examples of these traits include, but are not limited to,herbicide resistance, resistance to bacterial, fungal, or viral disease,insect resistance, modified fatty acid or carbohydrate metabolism, andaltered nutritional quality. These comprise genes generally inheritedthrough the nucleus.

Direct selection may be applied where the single locus acts as adominant trait. For this selection process, the progeny of the initialcross are assayed for viral resistance and/or the presence of thecorresponding gene prior to the backcrossing. Selection eliminates anyplants that do not have the desired gene and resistance trait, and onlythose plants that have the trait are used in the subsequent backcross.This process is then repeated for all additional backcross generations.

Selection of tomato plants for breeding is not necessarily dependent onthe phenotype of a plant and instead can be based on geneticinvestigations. For example, one can utilize a suitable genetic markerwhich is closely genetically linked to a trait of interest. One of thesemarkers can be used to identify the presence or absence of a trait inthe offspring of a particular cross, and can be used in selection ofprogeny for continued breeding. This technique is commonly referred toas marker assisted selection. Any other type of genetic marker or otherassay which is able to identify the relative presence or absence of atrait of interest in a plant can also be useful for breeding purposes.Procedures for marker assisted selection are well known in the art. Suchmethods will be of particular utility in the case of recessive traitsand variable phenotypes, or where conventional assays may be moreexpensive, time consuming or otherwise disadvantageous. Types of geneticmarkers which could be used in accordance with the invention include,but are not necessarily limited to, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990),Randomly Amplified Polymorphic DNAs (RAPDs), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified FragmentLength Polymorphisms (AFLPs) (EP 534 858, specifically incorporatedherein by reference in its entirety), and Single NucleotidePolymorphisms (SNPs) (Wang et al., Science, 280:1077-1082, 1998).

F. PLANTS DERIVED BY GENETIC ENGINEERING

Many useful traits that can be introduced by backcrossing, as well asdirectly into a plant, are those which are introduced by genetictransformation techniques. Genetic transformation may therefore be usedto insert a selected transgene into a plant of the invention or may,alternatively, be used for the preparation of transgenes which can beintroduced by backcrossing. Methods for the transformation of plantsthat are well known to those of skill in the art and applicable to manycrop species include, but are not limited to, electroporation,microprojectile bombardment, Agrobacterium-mediated transformation anddirect DNA uptake by protoplasts.

To effect transformation by electroporation, one may employ eitherfriable tissues, such as a suspension culture of cells or embryogeniccallus or alternatively one may transform immature embryos or otherorganized tissue directly. In this technique, one would partiallydegrade the cell walls of the chosen cells by exposing them topectin-degrading enzymes (pectolyases) or mechanically wound tissues ina controlled manner.

An efficient method for delivering transforming DNA segments to plantcells is microprojectile bombardment. In this method, particles arecoated with nucleic acids and delivered into cells by a propellingforce. Exemplary particles include those comprised of tungsten,platinum, and preferably, gold. For the bombardment, cells in suspensionare concentrated on filters or solid culture medium. Alternatively,immature embryos or other target cells may be arranged on solid culturemedium. The cells to be bombarded are positioned at an appropriatedistance below the macroprojectile stopping plate.

An illustrative embodiment of a method for delivering DNA into plantcells by acceleration is the Biolistics Particle Delivery System, whichcan be used to propel particles coated with DNA or cells through ascreen, such as a stainless steel or Nytex screen, onto a surfacecovered with target cells. The screen disperses the particles so thatthey are not delivered to the recipient cells in large aggregates.Microprojectile bombardment techniques are widely applicable, and may beused to transform virtually any plant species.

Agrobacterium-mediated transfer is another widely applicable system forintroducing gene loci into plant cells. An advantage of the technique isthat DNA can be introduced into whole plant tissues, thereby bypassingthe need for regeneration of an intact plant from a protoplast. ModernAgrobacterium transformation vectors are capable of replication in E.coli as well as Agrobacterium, allowing for convenient manipulations(Klee et al., Bio-Technology, 3(7):637-642, 1985). Moreover, recenttechnological advances in vectors for Agrobacterium-mediated genetransfer have improved the arrangement of genes and restriction sites inthe vectors to facilitate the construction of vectors capable ofexpressing various polypeptide coding genes. The vectors described haveconvenient multi-linker regions flanked by a promoter and apolyadenylation site for direct expression of inserted polypeptidecoding genes. Additionally, Agrobacterium containing both armed anddisarmed Ti genes can be used for transformation.

In those plant strains where Agrobacterium-mediated transformation isefficient, it is the method of choice because of the facile and definednature of the gene locus transfer. The use of Agrobacterium-mediatedplant integrating vectors to introduce DNA into plant cells is wellknown in the art (Fraley et al., Bio/Technology, 3:629-635, 1985; U.S.Pat. No. 5,563,055).

Transformation of plant protoplasts also can be achieved using methodsbased on calcium phosphate precipitation, polyethylene glycol treatment,electroporation, and combinations of these treatments (see, e.g.,Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985; Omirulleh et al.,Plant Mol. Biol., 21(3):415-428, 1993; Fromm et al., Nature,312:791-793, 1986; Uchimiya et al., Mol. Gen. Genet., 204:204, 1986;Marcotte et al., Nature, 335:454, 1988). Transformation of plants andexpression of foreign genetic elements is exemplified in Choi et al.(Plant Cell Rep., 13: 344-348, 1994), and Ellul et al. (Theor. Appl.Genet., 107:462-469, 2003).

A number of promoters have utility for plant gene expression for anygene of interest including but not limited to selectable markers,scoreable markers, genes for pest tolerance, disease resistance,nutritional enhancements and any other gene of agronomic interest.Examples of constitutive promoters useful for plant gene expressioninclude, but are not limited to, the cauliflower mosaic virus (CaMV)P-35S promoter, which confers constitutive, high-level expression inmost plant tissues (see, e.g., Odel et al., Nature, 313:810, 1985),including in monocots (see, e.g., Dekeyser et al., Plant Cell, 2:591,1990; Terada and Shimamoto, Mol. Gen. Genet., 220:389, 1990); a tandemlyduplicated version of the CaMV 35S promoter, the enhanced 35S promoter(P-e35S); 1 the nopaline synthase promoter (An et al., Plant Physiol.,88:547, 1988); the octopine synthase promoter (Fromm et al., Plant Cell,1:977, 1989); and the figwort mosaic virus (P-FMV) promoter as describedin U.S. Pat. No. 5,378,619 and an enhanced version of the FMV promoter(P-eFMV) where the promoter sequence of P-FMV is duplicated in tandem;the cauliflower mosaic virus 19S promoter; a sugarcane bacilliform viruspromoter; a commelina yellow mottle virus promoter; and other plant DNAvirus promoters known to express in plant cells.

A variety of plant gene promoters that are regulated in response toenvironmental, hormonal, chemical, and/or developmental signals can alsobe used for expression of an operably linked gene in plant cells,including promoters regulated by (1) heat (Callis et al., PlantPhysiol., 88:965, 1988), (2) light (e.g., pea rbcS-3A promoter,Kuhlemeier et al., Plant Cell, 1:471, 1989; maize rbcS promoter,Schaffner and Sheen, Plant Cell, 3:997, 1991; or chlorophyll a/b-bindingprotein promoter, Simpson et al., EMBO J., 4:2723, 1985), (3) hormones,such as abscisic acid (Marcotte et al., Plant Cell, 1:969, 1989), (4)wounding (e.g., wunl, Siebertz et al., Plant Cell, 1:961, 1989); or (5)chemicals such as methyl jasmonate, salicylic acid, or Safener. It mayalso be advantageous to employ organ-specific promoters (e.g., Roshal etal., EMBO J., 6:1155, 1987; Schernthaner et al., EMBO J., 7:1249, 1988;Bustos et al., Plant Cell, 1:839, 1989).

Exemplary nucleic acids which may be introduced to plants of thisinvention include, for example, DNA sequences or genes from anotherspecies, or even genes or sequences which originate with or are presentin the same species, but are incorporated into recipient cells bygenetic engineering methods rather than classical reproduction orbreeding techniques. However, the term “exogenous” is also intended torefer to genes that are not normally present in the cell beingtransformed, or perhaps simply not present in the form, structure, etc.,as found in the transforming DNA segment or gene, or genes which arenormally present and that one desires to express in a manner thatdiffers from the natural expression pattern, e.g., to over-express.Thus, the term “exogenous” gene or DNA is intended to refer to any geneor DNA segment that is introduced into a recipient cell, regardless ofwhether a similar gene may already be present in such a cell. The typeof DNA included in the exogenous DNA can include DNA which is alreadypresent in the plant cell, DNA from another plant, DNA from a differentorganism, or a DNA generated externally, such as a DNA sequencecontaining an antisense message of a gene, or a DNA sequence encoding asynthetic or modified version of a gene.

Many hundreds if not thousands of different genes are known and couldpotentially be introduced into a tomato plant according to theinvention. Non-limiting examples of particular genes and correspondingphenotypes one may choose to introduce into a tomato plant include oneor more genes for insect tolerance, such as a Bacillus thuringiensis(B.t.) gene, pest tolerance such as genes for fungal disease control,herbicide tolerance such as genes conferring glyphosate tolerance, andgenes for quality improvements such as yield, nutritional enhancements,environmental or stress tolerances, or any desirable changes in plantphysiology, growth, development, morphology or plant product(s). Forexample, structural genes would include any gene that confers insecttolerance including but not limited to a Bacillus insect control proteingene as described in WO 99/31248, herein incorporated by reference inits entirety, U.S. Pat. No. 5,689,052, herein incorporated by referencein its entirety, U.S. Pat. Nos. 5,500,365 and 5,880,275, hereinincorporated by reference in their entirety. In another embodiment, thestructural gene can confer tolerance to the herbicide glyphosate asconferred by genes including, but not limited to Agrobacterium strainCP4 glyphosate resistant EPSPS gene (aroA:CP4) as described in U.S. Pat.No. 5,633,435, herein incorporated by reference in its entirety, orglyphosate oxidoreductase gene (GOX) as described in U.S. Pat. No.5,463,175, herein incorporated by reference in its entirety.

Alternatively, the DNA coding sequences can affect these phenotypes byencoding a non-translatable RNA molecule that causes the targetedinhibition of expression of an endogenous gene, for example viaantisense- or cosuppression-mediated mechanisms (see, for example, Birdet al., Biotech. Gen. Engin. Rev., 9:207, 1991). The RNA could also be acatalytic RNA molecule (i.e., a ribozyme) engineered to cleave a desiredendogenous mRNA product (see for example, Gibson and Shillito, Mol.Biotech., 7:125, 1997). Thus, any gene which produces a protein or mRNAwhich expresses a phenotype or morphology change of interest is usefulfor the practice of the present invention.

G. DEFINITIONS

In the description and tables herein, a number of terms are used. Inorder to provide a clear and consistent understanding of thespecification and claims, the following definitions are provided:

Allele: Any of one or more alternative forms of a gene locus, all ofwhich alleles relate to one trait or characteristic. In a diploid cellor organism, the two alleles of a given gene occupy corresponding locion a pair of homologous chromosomes.

Backcrossing: A process in which a breeder repeatedly crosses hybridprogeny, for example a first generation hybrid (F₁), back to one of theparents of the hybrid progeny. Backcrossing can be used to introduce oneor more single locus conversions from one genetic background intoanother.

Crossing: The mating of two parent plants.

Cross-pollination: Fertilization by the union of two gametes fromdifferent plants.

Diploid: A cell or organism having two sets of chromosomes.

Emasculate: The removal of plant male sex organs or the inactivation ofthe organs with a cytoplasmic or nuclear genetic factor or a chemicalagent conferring male sterility.

Enzymes: Molecules which can act as catalysts in biological reactions.

F₁ Hybrid: The first generation progeny of the cross of two nonisogenicplants.

Genotype: The genetic constitution of a cell or organism.

Haploid: A cell or organism having one set of the two sets ofchromosomes in a diploid.

Linkage: A phenomenon wherein alleles on the same chromosome tend tosegregate together more often than expected by chance if theirtransmission was independent.

Marker: A readily detectable phenotype, preferably inherited incodominant fashion (both alleles at a locus in a diploid heterozygoteare readily detectable), with no environmental variance component, i.e.,heritability of 1.

Phenotype: The detectable characteristics of a cell or organism, whichcharacteristics are the manifestation of gene expression.

Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer togenetic loci that control to some degree numerically representabletraits that are usually continuously distributed.

Resistance: As used herein, the terms “resistance” and “tolerance” areused interchangeably to describe plants that show no symptoms to aspecified biotic pest, pathogen, abiotic influence or environmentalcondition. These terms are also used to describe plants showing somesymptoms but that are still able to produce marketable product with anacceptable yield. Some plants that are referred to as resistant ortolerant are only so in the sense that they may still produce a crop,even though the plants are stunted and the yield is reduced.

Regeneration: The development of a plant from tissue culture.

Royal Horticultural Society (RHS) color chart value: The RHS color chartis a standardized reference which allows accurate identification of anycolor. A color's designation on the chart describes its hue, brightnessand saturation. A color is precisely named by the RHS color chart byidentifying the group name, sheet number and letter, e.g., Yellow-OrangeGroup 19A or Red Group 41B.

Self-pollination: The transfer of pollen from the anther to the stigmaof the same plant.

Single Locus Converted (Conversion) Plant: Plants which are developed bya plant breeding technique called backcrossing, wherein essentially allof the morphological and physiological characteristics of a tomatovariety are recovered in addition to the characteristics of the singlelocus transferred into the variety via the backcrossing technique and/orby genetic transformation.

Substantially Equivalent: A characteristic that, when compared, does notshow a statistically significant difference (e.g., p=0.05) from themean.

Tissue Culture: A composition comprising isolated cells of the same or adifferent type or a collection of such cells organized into parts of aplant.

Transgene: A genetic locus comprising a sequence which has beenintroduced into the genome of a tomato plant by transformation.

H. DEPOSIT INFORMATION

A deposit of tomato hybrid SV4725TD and inbred parent lines FDR-9Q08131and FDR-9Q09139, disclosed above and recited in the claims, has beenmade with the American Type Culture Collection (ATCC), 10801 UniversityBlvd., Manassas, Va. 20110-2209. The date of deposits were Sep. 16,2013, Feb. 8, 2011, and Sep. 16, 2013, respectively. The accessionnumbers for those deposited seeds of tomato hybrid SV4725TD and inbredparent lines FDR-9Q08131 and FDR-9Q09139 are ATCC Accession No.PTA-120590, ATCC Accession No. PTA-11672, and ATCC Accession No.PTA-120592, respectively. Upon issuance of a patent, all restrictionsupon the deposits will be removed, and the deposits are intended to meetall of the requirements of 37 C.F.R. §1.801-1.809. The deposits will bemaintained in the depository for a period of 30 years, or 5 years afterthe last request, or for the effective life of the patent, whichever islonger, and will be replaced if necessary during that period.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

All references cited herein are hereby expressly incorporated herein byreference.

What is claimed is:
 1. A tomato plant comprising at least a first set ofthe chromosomes of tomato line FDR-9Q09139, a sample of seed of saidline having been deposited under ATCC Accession Number PTA-120592.
 2. Aseed comprising at least a first set of the chromosomes of tomato lineFDR-9Q09139, a sample of seed of said line having been deposited underATCC Accession Number PTA-120592.
 3. The plant of claim 1, which is aninbred.
 4. The plant of claim 1, which is a hybrid.
 5. The seed of claim2, which is an inbred.
 6. The seed of claim 2, which is a hybrid.
 7. Theplant of claim 4, wherein the hybrid plant is tomato hybrid SV4725TD, asample of seed of said hybrid SV4725TD having been deposited under ATCCAccession Number PTA-120590.
 8. The seed of claim 6, defined as a seedof tomato hybrid SV4725TD, a sample of seed of said hybrid SV4725TDhaving been deposited under ATCC Accession Number PTA-120590.
 9. Theseed of claim 2, defined as a seed of line FDR-9Q09139.
 10. A plant partof the plant of claim
 1. 11. The plant part of claim 10, further definedas a leaf, an ovule, pollen, a fruit, or a cell.
 12. A tomato planthaving all the physiological and morphological characteristics of thetomato plant of claim
 7. 13. A tissue culture of regenerable cells ofthe plant of claim
 1. 14. The tissue culture according to claim 13,comprising cells or protoplasts from a plant part selected from thegroup consisting of embryos, meristems, cotyledons, pollen, leaves,anthers, roots, root tips, pistil, flower, seed and stalks.
 15. A tomatoplant regenerated from the tissue culture of claim
 13. 16. A method ofvegetatively propagating the tomato plant of claim 1 comprising thesteps of: (a) collecting tissue capable of being propagated from theplant according to claim 1; (b) cultivating said tissue to obtainproliferated shoots; and (c) rooting said proliferated shoots to obtainrooted plantlets.
 17. The method of claim 16, further comprising growingat least a first tomato plant from said rooted plantlets.
 18. A methodof introducing a desired trait into a tomato line comprising: (a)crossing a plant of line FDR-9Q09139 with a second tomato plant thatcomprises a desired trait to produce F1 progeny, a sample of seed ofsaid line having been deposited under ATCC Accession Number PTA-120592;(b) selecting an F1 progeny that comprises the desired trait; (c)backcrossing the selected F1 progeny with a plant of line FDR-9Q09139 toproduce backcross progeny; (d) selecting backcross progeny comprisingthe desired trait and the physiological and morphological characteristicof tomato line FDR-9Q09139; and (e) repeating steps (c) and (d) three ormore times to produce selected fourth or higher backcross progeny thatcomprise the desired trait.
 19. A tomato plant produced by the method ofclaim
 18. 20. A method of producing a tomato plant comprising an addedtrait, the method comprising introducing a transgene conferring thetrait into a plant of tomato hybrid SV4725TD, or tomato lineFDR-9Q09139, a sample of seed of said hybrid and line having beendeposited under ATCC Accession Number PTA-120590, and ATCC AccessionNumber PTA-120592, respectively.
 21. A plant produced by the method ofclaim
 20. 22. The plant of claim 1, further comprising a transgene. 23.The plant of claim 22, wherein the transgene confers a trait selectedfrom the group consisting of male sterility, herbicide tolerance, insectresistance, pest resistance, disease resistance, modified fatty acidmetabolism, environmental stress tolerance, modified carbohydratemetabolism and modified protein metabolism.
 24. The plant of claim 1,further comprising a single locus conversion.
 25. The plant of claim 24,wherein the single locus conversion confers a trait selected from thegroup consisting of male sterility, herbicide tolerance, insectresistance, pest resistance, disease resistance, modified fatty acidmetabolism, environmental stress tolerance, modified carbohydratemetabolism and modified protein metabolism.
 26. A method for producing aseed of a tomato plant derived from at least one of tomato hybridSV4725TD, or tomato line FDR-9Q09139 comprising the steps of: (a)crossing a tomato plant of hybrid SV4725TD, or line FDR-9Q09139 withitself or a second tomato plant; a sample of seed of said hybrid andline having been deposited under ATCC Accession Number PTA-120590, andATCC Accession Number PTA-120592, respectively; and (b) allowing seed ofa hybrid SV4725TD, or line FDR-9Q09139-derived tomato plant to form. 27.The method of claim 26, further comprising the steps of: (c) selfing aplant grown from said hybrid SV4725TD, or FDR-9Q09139-derived tomatoseed to yield additional hybrid SV4725TD, or line FDR-9Q09139-derivedtomato seed; (d) growing said additional hybrid SV4725TD, or lineFDR-9Q09139-derived tomato seed of step (c) to yield additional hybridSV4725TD, or line FDR-9Q09139-derived tomato plants; and (e) repeatingthe crossing and growing steps of (c) and (d) to generate at least afirst further hybrid SV4725TD, or line FDR-9Q09139-derived tomato plant.28. The method of claim 26, wherein the second tomato plant is of aninbred tomato line.
 29. The method of claim 26, comprising crossing lineFDR-9Q09139 with line FDR-9Q08131, a sample of seed of said lines havingbeen deposited under ATCC Accession Number PTA-120592, and ATCCAccession Number PTA-11672, respectively.
 30. The method of claim 27,further comprising: (f) crossing the further hybrid SV4725TD, orFDR-9Q09139-derived tomato plant with a second tomato plant to produceseed of a hybrid progeny plant.
 31. A plant part of the plant of claim7.
 32. The plant part of claim 31, further defined as a leaf, an ovule,pollen, a fruit, or a cell.
 33. A method of producing a tomato seedcomprising crossing the plant of claim 1 with itself or a second tomatoplant and allowing seed to form.
 34. A method of producing a tomatocomprising: (a) obtaining the plant according to claim 1, wherein theplant has been cultivated to maturity; and (b) collecting a tomato fromthe plant.
 35. A method of producing a plant of tomato hybrid SV4725TDcomprising a single locus conversion, the method comprising crossing aplant of line FDR-9Q08131 with a plant of line FDR-9Q09139, wherein atleast one of said lines comprises a single locus conversion, a sample ofseed of said lines FDR-9Q08131 and FDR-9Q09139 having been depositedunder ATCC Accession No. PTA-11672 and ATCC Accession Number PTA-120592,respectively.